A Simple Metachromatic and Fluorescent Staining Method for Microorganisms Using Carbocyanine Dye
نویسندگان
چکیده
منابع مشابه
Metachromatic Granules of Microorganisms'
The exact nature and function of the metachromatic (volutin) granules seen in many microorganisms is still a matter of conjecture although polymetaphosphates (polymers of highenergy anhydride-linked phosphates) are a known constituent. The object of this paper is to re-examine volutin as a cytological entity, and to present a working hypothesis on the mechanism of formation of this substance. T...
متن کاملMetachromatic Granules of Microorganisms'
The exact nature and function of the metachromatic (volutin) granules seen in many microorganisms is still a matter of conjecture although polymetaphosphates (polymers of highenergy anhydride-linked phosphates) are a known constituent. The object of this paper is to re-examine volutin as a cytological entity, and to present a working hypothesis on the mechanism of formation of this substance. T...
متن کاملMetachromatic granules of microorganisms.
The exact nature and function of the metachromatic (volutin) granules seen in many microorganisms is still a matter of conjecture although polymetaphosphates (polymers of highenergy anhydride-linked phosphates) are a known constituent. The object of this paper is to re-examine volutin as a cytological entity, and to present a working hypothesis on the mechanism of formation of this substance. T...
متن کاملFluorescent Staining Method for Bacterial Endospores
We have developed a quick, simple technique for the fluorescent staining of bacterial endospores. This technique was developed using Bacillus subtilis endospores. Our technique requires minimal sample heating so it can safely be carried out entirely in a glove-box. The endospores stain diagnostically as an oval with a standard fluorescent nucleotide stain, acridine orange.
متن کاملStaining and peeling of the internal limiting membrane using a fluorescent dye (Rhodamine 6 G).
AIM To assess whether low concentrations of a fluorescent dye such as Rhodamine 6G would help the unaided human eye visualise the vitreous and the internal limiting membrane (ILM) under standard halogen illumination. MATERIAL/METHODS The UV/Vis absorption (E) and fluorescence (I) spectra of Rhodamine 6G in water were measured and compared with Indocyanine Green (ICG). Surgery was performed in...
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ژورنال
عنوان ژورنال: Microbiology and Immunology
سال: 1987
ISSN: 0385-5600
DOI: 10.1111/j.1348-0421.1987.tb03074.x